ForeDirect RT-qPCR Apo
Apejuwe Apo:
◮Fun imudara akoko gidi RNA taara lati awọn ikojọpọ swab laisi awọn ilana isọdọmọ RNA.Ohun elo yii pari awọn iyipo qRT-PCR laarin wakati kan.Iparapọ enzymu jẹ idapọ iṣapeye ti Reverse Transcriptase, Hot-Start Taq DNA polymerase, inhibitor RNase.Idaduro Idahun ni gbogbo awọn paati ti a beere, pẹlu iṣapeye awọn paati ifipamọ, Mg2+, dUTP, ati dNTPs.
Apejuwe
Apo ForeDirect RT-qPCR jẹ apẹrẹ fun imudara akoko gidi RNA taara lati awọn ikojọpọ swab laisi awọn ilana isọdọmọ RNA.Ohun elo yii pari awọn iyipo qRT-PCR laarin wakati kan.Iparapọ enzymu jẹ idapọ iṣapeye ti Reverse Transcriptase, Hot-Start Taq DNA polymerase, inhibitor RNase.Idaduro Idahun ni gbogbo awọn paati ti a beere, pẹlu iṣapeye awọn paati ifipamọ, Mg2+, dUTP, ati dNTPs.
Awọn pato
100T
Awọn paati ohun elo
| Nucleic acid oluranlowo itusilẹ |
| RNA aabo |
| 2× RT-qPCR saarin |
| Àdàpọ̀ Enzyme (Taq&M-MLV) |
| Awọn ilana |
PCR Ohun elo
1. Ni kete ti awọn tubes ifaseyin PCR ti ni centrifuged ni ṣoki, gbe wọn sinu ojò apẹẹrẹ ti ohun elo imudara.
2. Gbona Ilana
| Igbesẹ | Iwọn otutu | Aago | Awọn iyipo | |
| 1 | Yiyipada transcription | 50℃ | 15 iṣẹju | 1 |
| 2 | Pre-denaturation | 95℃ | 1 iseju | 1 |
| 3 | Denaturation | 95℃ | 10 aaya | 42 |
| 4 | Anneal / Ifaagun | 60℃ | 30 aaya | |
Akiyesi:A ṣe akiyesi ifihan agbara Fuluorisenti lẹsẹkẹsẹ lẹhin igbesẹ itẹsiwaju ti ọmọ kọọkan.
3. Lẹhin ti eto, fi awọn faili ati ṣiṣe awọn lenu eto.
Awọn Igbesẹ Ṣiṣẹ
Ṣaaju igbaradi reagent, gbogbo awọn reagents ninu ohun elo yẹ ki o jẹ thawed ni iwọn otutu yara ati dapọ rọra.
Reagent igbaradi
1. Mura Apapo Acid Acid Nucleic
| Ẹya ara ẹrọ | Iwọn didun fun Ayẹwo tabi Iṣakoso |
| Nucleic acid oluranlowo itusilẹ | 5μL |
| RNA aabo | 0.5 μL |
2. Mura Apapo Iṣe
| Ẹya ara ẹrọ | Iwọn didun fun Ayẹwo tabi Iṣakoso |
| 2× RT-qPCR saarin | 15 μL |
| Àdàpọ̀ Enzyme (Taq&M-MLV) | 1.5 μL |
| Alakoko ati wadi | 1μL |
Ibi ipamọ ati igbesi aye Selifu
-Awọn kit yẹ ki o wa ni ipamọ ni -20± 5℃.Awọn Wiwulo akoko ni 12 osu.
Yẹra fun awọn iyipo didi-diẹ leralera (<5 cycles).
