Ohun elo Iwari Acid Nucleic Acid SARS-CoV-2 (Ọna Iwadi Fluorescent PCR Multiplex)
Lilo ti a pinnu
Ohun elo yii nlo imọ-ẹrọ RT PCR gidi-akoko (rRT-PCR) fun wiwa agbara ti awọn acids nucleic SARS-CoV-2 ninu awọn ayẹwo nasopharyngeal eniyan tabi awọn ayẹwo swab oropharyngeal.
Ilana ti idanwo
Alakoko yii ati awọn eto iwadii jẹ apẹrẹ fun wiwa ti awọn ilana ti a fipamọ ti SARS-CoV-2 (jiini ORF1ab, N pupọ ati jiini E).Ohun elo naa ni Iṣakoso Inu kan ti a lo lati ṣe ayẹwo didara apẹrẹ.Imọ-ẹrọ PCR akoko-gidi nlo iṣesi pq polymerase (PCR) fun imudara awọn ilana ibi-afẹde kan pato ati ibi-afẹde kan pato fun wiwa ti RNA imudara.Awọn iwadii naa jẹ aami pẹlu onirohin Fuluorisenti ati awọn awọ parun.
Awọn alaye kiakia
◮Awọn pato:48 Rxns / ohun elo, 96 Rxns / ohun elo
Ni pato:96.72%
Ifamọ:97.92%
◮ Awọn iru apẹrẹ ti o wulo
Nasopharyngeal Swab tabi Oropharyngeal Swab
◮Ohun elo to wulo
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S
Awọn eroja
Rara. | Ẹya ara ẹrọ | Iye | Eroja akọkọ | |||
48 Rxn | 96 Rxn | |||||
1 | Nucleic acid itusilẹoluranlowo | 1,5 milimita / tube | tube 1 | 1,5 milimita / tube | tube 1 | Surfactant |
2 | RNA aabo | 27 μL / tube | tube 1 | 53 μL / tube | tube 1 | RNase onidalẹkun |
3 | SARS-CoV-2lenu ojutu | 800 μL / tube | tube 1 | 1600 μL / tube | tube 1 | Alakoko, iwadii, ifasilẹ esi, dNTP |
4 | SARS-CoV-2enzymu adalu | 80 μL / tube | tube 1 | 160 μL / tube | tube 1 | Ibẹrẹ gbigbona Taq enzyme,M-MLV enzymu |
5 | SARS-CoV-2rere Iṣakoso | 100 μL / tube | tube 1 | 100 μL / tube | tube 1 | Atunkọ pilasimadi ti o ni ibi-afẹdeajeku, RNA |
6 | SARS-CoV-2odi Iṣakoso | 1200 μL / tube | tube 1 | 1200 μL / tube | tube 1 | TE ifipamọ |
Awọn ẹya & awọn anfani
◮PCR taara
Ko si iwulo fun ohun elo imukuro acid nucleic ati Eto, ti o pari apẹrẹ 96 ni wakati kan tabi bẹẹbẹẹ.
◮Ibeere ohun elo kekere ati lilo irọrun pupọ
Nikan nilo Real-Time PCR eto.
◮LoD ati ki o ga ifamọ
Ifamọ wiwa le de ipele ti o kere julọ bi awọn adakọ 500 / milimita.
Bawo ni lati lo
Ọna 1: Ọna PCR taara
Ọna 2: Gbogun ti RNA Iyasọtọ + PCR
Ọna 1: Taara PCR ọna liloAṣoju Tu Ayẹwo
Tutu pq Transportation ati Ibi ipamọ
Ididi lati ina ati ti o ti fipamọ ni -20 ± 5 ℃;
Igbesi aye selifu
1 odun
Bere fun Alaye
Fidio Isẹ kiakia
Kọ ifiranṣẹ rẹ nibi ki o si fi si wa