Ẹjẹ SuperDirectᵀᴹ PCR Kit-Heparin Ẹjẹ Taara PCR Master Mix fun Jinotyping ti Ẹjẹ
Awọn pato
200 ×20μl rxns, 2000 ×20μl rxns
2× SuperEasyTMMix-Heparin: Ni awọn iyipada pataki D-Taq DNA Polymerase, dNTPs, MgCl2, ifasilẹ ifasilẹ, imudara esi PCR, olupilẹṣẹ, imuduro, bbl Ninu ifa PCR, kan ṣafikun gbogbo ẹjẹ anticoagulated ti o yẹ, awọn alakoko, ati ddH2Eyin si 2× SuperEasy ti o baamuTMMix-Heparin lati ṣee lo ninu iṣesi PCR.
6× DNA Loading Buffer: Ifipamọ Ikojọpọ ko ni SDS ninu.A gba ọ niyanju lati lo 6 × DNA Loading Buffer ti o wa pẹlu ohun elo nigbati o ba n ṣe elerophoresis gel agarose lati le gba awọn abajade elekitirophoresis to dara.Ma ṣe lo Ifipamọ Loading ti o ni SDS, bibẹẹkọ iru nla ti ina didan yoo wa ni oju ọna lakoko electrophoresis, eyiti yoo kan awọn abajade esiperimenta.
Awọn paati ohun elo
2× SuperEasyTM Mix-Heparin |
6× DNA Loading saarin |
Awọn ilana |
Awọn ẹya & awọn anfani
-Ko si nilo fun akoko-n gba ati ki o gbowolori DNA ìwẹnumọ, ko si pretreatment, ati taara lilo ti gbogbo ẹjẹ bi a PCR awoṣe.
-Eto naa ni agbara imudara ti o lagbara ati pe o le ṣe akiyesi jiini ati awọn ajẹku DNA ibi-afẹde ajeji ninu ẹjẹ.
-2× SuperEasyTMMix-Heparin Ifarada ẹjẹ giga: to 45% ninu ẹjẹ eniyan ati 20% ninu ẹjẹ Asin.
Ayẹwo naa ti wa ni pipade ni kikun, nitorinaa ko si iwulo lati ṣe aniyan nipa ibajẹ ayẹwo ati awọn abajade PCR rere eke.
Ohun elo Kit
Igbẹhin lati ṣe idanimọ PCR taara ti Heparin anti-coagulated gbogbo ẹjẹ.(Pẹlu: eje eniyan, eje eku, eje adie, eje eye, eje maalu, eje aja, ati be be lo)
Ṣiṣan iṣẹ
Ibi ipamọ ati igbesi aye Selifu
Ohun elo yii yẹ ki o wa ni ipamọ ni -20 ℃.
2× SuperEasyTMMix-Heparin, Ti o ba lo nigbagbogbo, o le wa ni ipamọ ni 4 ° C fun ibi ipamọ igba kukuru (lo laarin awọn ọjọ 10).
6 × DNA Loading Buffer, O le wa ni ipamọ ni 4°C tabi -20°C fun igba pipẹ.