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asia_oju-iwe

Apapọ Apapọ Ẹranko Ohun elo Ipinya RNA Lapapọ Iyọkuro RNA ati Awọn ohun elo Iwẹnumọ fun Awọn sẹẹli Ẹranko & Ẹnu

Apejuwe Kit:

Ni kiakia ati daradara jade ni mimọ-giga ati didara lapapọ RNA lati ọpọlọpọ awọn ẹran ara ẹranko.

Ko si ye lati ṣe aniyan nipa ibajẹ RNA.Gbogbo eto jẹ Ọfẹ RNase

Yọ DNA kuro ni imunadoko nipa lilo DNA-Cleaning Column

Yọ DNA kuro laisi fifi DNAse kun

Rọrun-gbogbo awọn iṣẹ ṣiṣe ti pari ni iwọn otutu yara

Yara-iṣiṣẹ le pari ni ọgbọn iṣẹju

Ailewu-ko si reagent Organic ti a lo

Iwa mimọ-OD260/280≈1.8-2.1

foregene agbara


Alaye ọja

ọja Tags

FAQ

Kits Awọn apejuwe

Awọn igbaradi 50, Awọn igbaradi 200

Ohun elo yii nloomo ọwọn ati agbekalẹti o ni idagbasoke nipasẹ ile-iṣẹ wa, eyiti o le jade ni mimọ-giga ati didara lapapọ RNA lati ọpọlọpọ awọn ẹran ara ẹranko pẹlu ṣiṣe giga.O pese iwe-itọju DNA ti o munadoko, eyiti o le ni rọọrun yapa ati adsorb DNA genomic lati supernatant ati lysate tissu, rọrun. ati fifipamọ akoko;Ọwọn RNA-nikan le di RNA daradara ati pe o le ṣe ilọsiwaju nigbakanna pẹlu agbekalẹ alailẹgbẹ Ọpọlọpọ awọn ayẹwo.

Gbogbo eto jẹ RNase-ọfẹ, ki RNA ti a fa jade ko ni ibajẹ;Buffer RW1, Buffer RW2 eto fifọ saarin, ki RNA ti o gba jẹ ọfẹ ti amuaradagba, DNA, ion, ati idoti agbo-ara Organic.

Awọn paati ohun elo

Animal Total RNA ipinya Apo
Awọn paati ohun elo RE-03011 RE-03014
50 T 200 T
Ifipamọ RL1* 25ml 100ml
Ifipamọ RL2 15ml 60ml
Ifipamọ RW1* 25ml 100ml
Ifipamọ RW2 24ml 96ml
RNase-Ọfẹ ddH2O 10 milimita 40ml
Ọwọn RNA-nikan 50 200
Ọwọn Isọmọ DNA 50 200
Ilana itọnisọna 1 nkan 1 nkan

 

ọja alaye

Ọna kika omo ere paati ìwẹnumọ Foregene ọwọn, reagent
Flux 1-24 awọn ayẹwo Akoko fun igbaradi ~ 30 iṣẹju (awọn ayẹwo 24)
Centrifuge Iduro centrifuge Pyrolysis Iyapa Centrifugal Iyapa
Apeere Ẹranko ẹran;sẹẹli Awọn ayẹwo iye Asọ: 10-20 mg;Ẹyin: (1-5)×106
Elution iwọn didun 50-200 μL Iwọn ikojọpọ ti o pọju 850 μL

 

Awọn ẹya & awọn anfani

■ Ko si ye lati ṣe aniyan nipa ibajẹ RNA;gbogbo eto jẹ RNase-ọfẹ
■ Yọ DNA kuro daradara-lilo DNA-Cleaning Column
■ Yọ DNA kuro lai fi DNAse kun
■ Rọrun-gbogbo awọn iṣẹ ṣiṣe ti pari ni iwọn otutu yara
■ Ṣiṣe-yara le pari ni ọgbọn išẹju 30
■ Ailewu-ko si reagenti Organic ti o nilo
■ Giga ti nw -OD260 / 280≈1.8-2.1

awọn anfani ti foregene RNA Isolation kit

Ohun elo Kit

O dara fun isediwon ati ìwẹnumọ ti lapapọ RNA lati ọpọlọpọ awọn ẹran ara tuntun tabi tutunini tabi awọn sẹẹli gbin.

Ọja sile

■ Awọn ohun elo ti o wa ni isalẹ: iṣakojọpọ cDNA akọkọ-okun, RT-PCR, cloning molikula, Northern Blot, ati bẹbẹ lọ.
■ Awọn ayẹwo: awọn ẹran ara ẹranko, awọn sẹẹli ti a gbin
■ Iwọn lilo: Tissues 10-20mg, Awọn sẹẹli (2-5) × 106
■ O pọju agbara abuda DNA ti ọwọn ìwẹnumọ: 80 μg
■ Iwọn didun: 50-200 μl

eranko lapapọ RNA-rọrun bisesenlo

Aworan atọka