Foregene DNA Idanimọ System 27Y
Apejuwe Apo:
Foregene Eto Idanimọ DNA 27Y nlo imọ-ẹrọ isamisi Fuluorisenti awọ mẹfa lati mu iwọn 27 Y-STR pọ si ni akoko kan.Pẹlu: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS448, DYS533, DYF387, DYS387 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
Awọn kit ni o ni ti o dara egboogi-idinamọ agbara, ati ki o le taara amplify àlẹmọ iwe, FTA kaadi, owu swab, itọ kaadi, roba swab, ati ki o le tun ti wa ni lo lati jade ki o si wẹ DNA awọn awoṣe lati irú awọn ohun elo.
Apejuwe
Foregene Eto Idanimọ DNA 27Y nlo imọ-ẹrọ isamisi Fuluorisenti awọ mẹfa lati mu iwọn 27 Y-STR pọ si ni akoko kan.Pẹlu: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS448, DYS533, DYF387, DYS387 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
Awọn kit ni o ni ti o dara egboogi-idinamọ agbara, ati ki o le taara amplify àlẹmọ iwe, FTA kaadi, owu swab, itọ kaadi, roba swab, ati ki o le tun ti wa ni lo lati jade ki o si wẹ DNA awọn awoṣe lati irú awọn ohun elo.
Akoonu Kit
| Ohun elo idanwo Reagent | Orukọ paati | Iwọn didun (μl/tube) | Opoiye (tube) |
| PCR ami-ifojusi kit | 2× PCR Reaction Titunto Mix | 1250 | 2 |
| 5 x 27Y Alakoko Mix | 500 | 2 | |
| Iwọn titẹ DNA 9948 | 20 | 2 | |
| Omi ti a fi omi ṣan | 1700 | 2 | |
| Post-PCR ifaseyin Kit | 27Y allele adalu akaba | 20 | 2 |
| Òṣuwọn molikula Orange boṣewa abẹnu ORG500 | 150 | 2 |
Data onínọmbà
1. Ṣii sọfitiwia ID Gene Mapper.Nigbati o ba nlo ohun elo yii fun igba akọkọ, o nilo lati gbe awọn panẹli, ni ọna BIL, 320, 10020, 390, 390, 320, 4000).
2. Gbe wọle data electrophoresis, yan awọn igbelewọn itupalẹ ti o baamu gẹgẹbi nronu, ọna itupalẹ, ati iwọn iwọn, ki o yi iru apẹẹrẹ ti Ladder pada si “Allelic Ladder” ni “Iru Ayẹwo” iwe;bẹrẹ itupalẹ data.
Ibi ipamọ Reagent
1. Jọwọ tọju wọn ni isalẹ -20 ° C (ti ko ba lo fun igba diẹ), lẹhin gbigba awọn ohun elo ti a ti didi ni yinyin gbigbẹ tabi awọn akopọ yinyin gel.
2. Jọwọ tọju ohun elo naa ni 4°C ṣaaju iṣesi lati yago fun didi ati didi leralera, lẹhin ti o ti gbe ohun elo naa jade fun lilo,;Enzymu yẹ ki o wa ni ipamọ ni -20 ° C.
2. Lẹhin ifasẹyin, kit naa (pẹlu iwuwo molikula ti inu iwọnwọn ati akaba) yẹ ki o wa ni ipamọ ni “yara wiwa elekitirogi” ni 4 ° C, yago fun didi ati didi leralera, ki o yago fun olubasọrọ pẹlu ohun elo ifasilẹ tẹlẹ lati yago fun idoti.
