SARS-CoV-2 Ohun elo Wiwa Acid Nucleic (Ọna Iwadi Fuluorisenti Multiplex PCR)

SARS-CoV-2 Ohun elo Wiwa Acid Nucleic (Ọna Iwadi Fuluorisenti Multiplex PCR)

Apejuwe Apo:

Ohun elo yii nlo imọ-ẹrọ RT PCR akoko-gidi (rRT-PCR) fun wiwa agbara ti SARS-CoV-2 (pupọ ORF1ab ati N pupọ) iṣakoso inu lati ṣe ayẹwo didara apẹrẹ, nitorinaa ko nilo fun isọdimimọ acid nucleic, le pari idanwo ni wakati 1, paapaa ti o yẹ fun wiwa dekun titobi.


Ọja Apejuwe

Ọja Tags

Ni pato

48 Rxns, 96 Rxns

Rara.

Paati

Iye

Main irinše

48 Rxns

96 Rxns

1 Oluranlowo idasilẹ Nucleic acid 1.4mL / tube Awọn tubes 2 5.3 milimita / igo 1 igo Surfactant
2 Olugbeja RNA 27 μL / tube 1 ọpọn 53 μL / tube 1 ọpọn Onidalẹkun RNase
3 SARS-CoV-2 ojutu ifaseyin 800 μL / tube 1 ọpọn 1600 μL / tube 1 ọpọn Alakoko, iwadii, ifipamọ ifura, dNTP
4 Apo enzymu SARS-CoV-2 80 μL / tube 1 ọpọn 160 μL / tube 1 ọpọn Ibẹrẹ gbigbona Taq henensiamu, enzymu M-MLV
5 SARS-CoV-2 iṣakoso rere 100 μL / tube 1 ọpọn 100 μL / tube 1 ọpọn Plasmid atunkun ti o ni abawọn ibi-afẹde, RNA
6 SARS-CoV-2 iṣakoso odi 1200 μL / tube 1 ọpọn 1200 μL / tube 1 ọpọn TE saarin

Awọn ẹya & awọn anfani

Ni kiakia ati irọrun.
Yago fun pipadanu acid nucleic.
Ifilelẹ wiwa kekere ati ifamọ giga.
Ibeere ohun elo kekere ati lilo rirọ pupọ.

Imọra erin le de ipele ti o kere julọ bi awọn ẹda 500 / milimita).

GCE / milimita Ct iye
N jiini Jiini ORF1ab
2.00E + 08 16.43 15.16
2.00E + 07 19.48 18.58
2.00E + 06 22.71 21.86
2.00E + 05 25.95 25.33
2.00E + 04 29.30 28.49
2.00E + 03 32.37 31.37
2.00E + 02 35.32 34.47
2.00E + 01 38.29 38.27
2.00E + 00 N / A N / A
2.00E-01 N / A N / A
NC N / A N / A

Iwọn titobi titobi Onitara

amplification curve-RT-qPCR

Bawo ni lati lo

1. Ohun elo ti o wulo

covid-19 nucleic acid detection kit1

2. Ilana wiwa

covid-19 nucleic acid detection kit2

Ṣiṣe ayẹwo: Naopharyngeal tabi orofaryngeal swab sample (iṣẹ igbesẹ 1 nikan) (Duro fun awọn iṣẹju 10)

Ampificaton PCR (<= 55min)

Bisesenlo:

covid-19 nucleic acid detection kit4

Ibi ipamọ

edidi lati ina ati fipamọ ni -20 ± 5 ℃;

storage
storage2

Aye igbesi aye: Ọdun 1


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