Taara RT-qPCR Apo
Apejuwe Apo:
◮ Apo RT-qPCR Taara, ni lilo Foreasy Reverse Transcriptase ati Foreasy HS Taq DNA Polymerase ti o dagbasoke nipasẹ Foregene, pẹlu ifipamọ ifa alailẹgbẹ, jẹ ki kit yii pẹlu resistance to lagbara ati ibaramu, ati pe o le ṣe idanwo esi taara lilo Foregene Lysis eto bi awoṣe.Ohun elo yii le waye fun ohun elo wiwa nucleic acid COVID-19 ati idagbasoke ohun elo wiwa acid nucleic miiran.
Apejuwe
Apo RT-qPCR Taara n pese ifipamọ RT-qPCR ti o papọ ati henensiamu to munadokoadalu (Taq, M-MLV, RI) ， jẹ ki o rọrun fun ṣiṣẹda awọn ohun elo ipari ati etotolesese, laarin rọrun ijerisi.
Awọn pato
500T, 50,000T
Awọn paati ohun elo
| Awọn akoonu inu ohun elo (20 μL eto esi) | IM-05111-01 | IM-05112-01 |
| 500 T | 50,000 T | |
| Adalu Enzymu Taara | 500 μL×1 | 50 milimita×1 |
| 2× Taara RT-qPCR saarin | 1 milimita×5 | 500 milimita×1 |
| Ilana | 1 | 1 |
Awọn Igbesẹ Ṣiṣẹ
Ṣaaju igbaradi reagent, gbogbo awọn reagents ninu ohun elo yẹ ki o jẹ thawed ni iwọn otutu yara ati dapọ rọra.
A: Murasilẹ of awoṣe ati reagenti
1. Mura awoṣe RNA ti a pese silẹ (A ṣe iṣeduro lati lo ohun elo Foregene Total RNA Isolation Kit jara lati yọ jade ati sọ awoṣe RNA di mimọ) tabi ọja fifọ ayẹwo (A ṣe iṣeduro lati lo Foregene Lysis eto), awọn alakoko kan pato (10 μM) ati awọn ohun elo miiran ti o yẹ, ohun elo.
2. Fi 2× Taara RT-qPCR Buffer, RNase-Free ddH2O ati 20× ROX Itọkasi Dye (ti o ba jẹ's nilo) ninu apoti pẹlu yinyin, ṣiṣe awọn wọnyi yo nipa ti ara, ati adalu rọra.
B: Murasilẹ of RT-qPCR eto
Tabili 1 : Murasilẹ of RT-qPCR seto
| Ẹya ara ẹrọ | Iwọn didun | Ifojusi ikẹhin |
| 2× Taara RT-qPCR saarin | 10 μL | 1× |
| Adalu Enzymu Taara | 1μL | |
| Alakoko siwaju (10 μM) | 0.8 μL | 50-900nM |
| Yipada alakoko (10 μM) | 0.8 μL | 50-900nM |
| Iwadi (4 μM) | 1μL | 200nM |
| Awoṣe (RNA tabi Lysate) | X μL | |
| 20× ROX Itọkasi Dye | - | 1* |
| RNase-FreeddH2O | (6.4-X) μL | |
| Lapapọ Iwọn didun | 20μL |
C: Ṣeto awọn RT-qPCR lenu eto
Tabili 2 : RT-qPCR fesion eto eto (fun apẹẹrẹ: meji-igbese ọna)
| Igbesẹ | Iwọn otutu | Aago | Awọn iyipo | Akoonu | |
| 1 | 50℃ | 15 min | 1* | 1 | Yiyipada transcription |
| 2 | 95℃ | 1 iseju | 1 | Pre-denaturation | |
| 3 | 95℃ | 10 iṣẹju-aaya | 40-45 | Denaturation | |
| 60℃ | iṣẹju-aaya 30 2* | Anneal / Ifaagun | |||
Ibi ipamọ ati igbesi aye Selifu
-Awọn kit yẹ ki o wa ni ipamọ ni -20± 5℃.Awọn Wiwulo akoko ni 12 osu.
Yẹra fun awọn iyipo didi-diẹ leralera (<5 cycles).
